C2331

Immunoblotting
CEA preparation extract was separated on SDS-PAGE and probed with Monoclonal AB To CEA (MO)CL:C6G9 (PREPK) (Cat. No. C2331). The antibody was developed with Alk Phos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate.
Antibody dilution 1/1500

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C2331

Sigma

 

Monoclonal Anti-Carcinoembryonic Antigen antibody produced in mouse

clone C6G9, ascites fluid

MDL number:MFCD00162210

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Description

Immunogencarcinoembryonic antigen (CEA) isolated from a human colon adenocarcinoma cell line.
SpecificityThe antibody reacts specifically with human CEA (CD66e, 180 kDa) from several types of malignant tissue including colorectal, lung and breast tumors. It also stains medullary carcinomas of the thyroid. The product reacts strongly with the cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. A weak reactivity is seen with normal colon mucosa and occasionally with bile canalicular and pancreatic acinar cells, but not with other tested tissue including term placenta. There is no cross-reactivity with non-specific cross-reacting antigen (NCA) present in granulocytes, using immunoblotting, flow cytometry and immunohistochemical techniques. The antibody recognizes an epitope that is resistant to 30 minute oxidation by 1% sodium periodate solution in routine formalin-fixed and paraffin-embedded tissue sections. Enzymatic predigestion with proteolytic enzymes improves immunohistochemical staining with the antibody.

Properties

antibody formascites fluid
cloneC6G9 (monoclonal)
contains15 mM sodium azide
species reactivityhuman
application(s)flow cytometry: suitable
 immunoblotting: suitable
 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:8,000 using human colon carcinoma tissue
isotypeIgG1
Gene Informationhuman ... CEACAM1(634)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3